AWtoolbox (Audio Word Toolbox) is an open-source software designed for extracting the audio word (AW) representation of audio signals. This document describes the usage of AWtoolbox for both basic users who are interested in extracting AW representation with the toolbox's graphical user interface and advanced users who are interested to learn about the details of audio word extraction process or to expend the functionality of the toolbox. Additionally, a preliminary benchmark on tweaking various components of audio word extraction process is presented. The preliminary benchmark shows how one can use AWtoolbox to procedurally search for the best AW representation for an audio recognition problem. AWtoolbox is available for download at http://mac.citi.sinica.edu. tw/awtoolbox.